41 research outputs found
Assessing DESS solution for the long-term preservation of nematodes from faecal samples
Preservation of biological samples is a relevant issue for many scientific disciplines. Although traditional preservers, such as formaldehyde or ethanol, imply major disadvantages related to health risks, DNA degradation and distortion of structures, they are widely used. Hence, the search for viable alternatives preserving morphometry and genetics seems necessary. Here we assess the suitability of DESS solution to preserve adult nematodes and their eggs in faeces. Concretely, faecal samples of terrestrial tortoises with oxyurids were used to: (i) compare the 1-month storage efficacy of eggs from different conservation protocols (faeces without preserver at -20 °C, faeces with DESS solution at room temperature, faeces with DESS solution at -20 °C and faeces with ethanol 70% at room temperature); (ii) address morphological nematode identification after 2 years of storage with DESS. We also corroborated that nematode DNA remained viable after 2 years. Overall, our results showed that DESS solution at room temperature is an advisable alternative to conserve both parasite eggs and adult nematodes for morphological identification and genetic purposes. It also offers the advantages of being low-cost, safe and suitable for fieldwork conditions and shipments without refrigeration for nematode preservation.MG was supported by a contract for postdoctoral researchers from the Generalitat Valenciana with reference APOSTD/2021/181. RCRC was supported by the European Union-Next Generation EU in the Maria Zambrano Program (ZAMBRANO 21-26). This research had the financial support of project PID2019-105682RA-I00, funded by the Spanish Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033
Radio continuum observations of Class I protostellar disks in Taurus: constraining the greybody tail at centimetre wavelengths
We present deep 1.8 cm (16 GHz) radio continuum imaging of seven young
stellar objects in the Taurus molecular cloud. These objects have previously
been extensively studied in the sub-mm to NIR range and their SEDs modelled to
provide reliable physical and geometrical parametres.We use this new data to
constrain the properties of the long-wavelength tail of the greybody spectrum,
which is expected to be dominated by emission from large dust grains in the
protostellar disk. We find spectra consistent with the opacity indices expected
for such a population, with an average opacity index of beta = 0.26+/-0.22
indicating grain growth within the disks. We use spectra fitted jointly to
radio and sub-mm data to separate the contributions from thermal dust and radio
emission at 1.8 cm and derive disk masses directly from the cm-wave dust
contribution. We find that disk masses derived from these flux densities under
assumptions consistent with the literature are systematically higher than those
calculated from sub-mm data, and meet the criteria for giant planet formation
in a number of cases.Comment: submitted MNRA
A blind detection of a large, complex, Sunyaev--Zel'dovich structure
We present an interesting Sunyaev-Zel'dovich (SZ) detection in the first of
the Arcminute Microkelvin Imager (AMI) 'blind', degree-square fields to have
been observed down to our target sensitivity of 100{\mu}Jy/beam. In follow-up
deep pointed observations the SZ effect is detected with a maximum peak
decrement greater than 8 \times the thermal noise. No corresponding emission is
visible in the ROSAT all-sky X-ray survey and no cluster is evident in the
Palomar all-sky optical survey. Compared with existing SZ images of distant
clusters, the extent is large (\approx 10') and complex; our analysis favours a
model containing two clusters rather than a single cluster. Our Bayesian
analysis is currently limited to modelling each cluster with an ellipsoidal or
spherical beta-model, which do not do justice to this decrement. Fitting an
ellipsoid to the deeper candidate we find the following. (a) Assuming that the
Evrard et al. (2002) approximation to Press & Schechter (1974) correctly gives
the number density of clusters as a function of mass and redshift, then, in the
search area, the formal Bayesian probability ratio of the AMI detection of this
cluster is 7.9 \times 10^4:1; alternatively assuming Jenkins et al. (2001) as
the true prior, the formal Bayesian probability ratio of detection is 2.1
\times 10^5:1. (b) The cluster mass is MT,200 = 5.5+1.2\times 10^14h-1M\odot.
(c) Abandoning a physical model with num- -1.3 70 ber density prior and instead
simply modelling the SZ decrement using a phenomenological {\beta}-model of
temperature decrement as a function of angular distance, we find a central SZ
temperature decrement of -295+36 {\mu}K - this allows for CMB primary
anisotropies, receiver -15 noise and radio sources. We are unsure if the
cluster system we observe is a merging system or two separate clusters.Comment: accepted MNRAS. 12 pages, 9 figure
AMI observations of unmatched Planck ERCSC LFI sources at 15.75 GHz
The Planck Early Release Compact Source Catalogue includes 26 sources with no
obvious matches in other radio catalogues (of primarily extragalactic sources).
Here we present observations made with the Arcminute Microkelvin Imager Small
Array (AMI SA) at 15.75 GHz of the eight of the unmatched sources at
declination > +10 degrees. Of the eight, four are detected and are associated
with known objects. The other four are not detected with the AMI SA, and are
thought to be spurious.Comment: 6 pages, 5 figures, 4 table
"Author! Author!" : Shakespeare and biography
Original article can be found at: http://www.informaworld.com/smpp/title~content=t714579626~db=all Copyright Informa / Taylor & Francis Group. DOI: 10.1080/17450910902764454Since 1996, not a year has passed without the publication of at least one Shakespeare biography. Yet for many years the place of the author in the practice of understanding literary works has been problematized, and even on occasions eliminated. Criticism reads the âworksâ, and may or may not refer to an author whose âlifeâ contributed to their meaning. Biography seeks the author in the works, the personality that precedes the works and gives them their characteristic shape and meaning. But the form of literary biography addresses the unusual kind of âlifeâ that puts itself into âworksâ, and this is particularly challenging where the âworksâ predominate massively over the salient facts of the âlifeâ. This essay surveys the current terrain of Shakespeare biography, and considers the key questions raised by the medium: can we know anything of Shakespeare's âpersonalityâ from the facts of his life and the survival of his works? What is the status of the kind of speculation that inevitably plays a part in biographical reconstruction? Are biographers in the end telling us as much about themselves as they tell us about Shakespeare?Peer reviewe
AMI-LA radio continuum observations of Spitzer c2d small clouds and cores: Perseus region
We present deep radio continuum observations of the cores identified as
deeply embedded young stellar objects in the Perseus molecular cloud by the
Spitzer c2d programme at a wavelength of 1.8 cm with the Arcminute Microkelvin
Imager Large Array (AMI-LA). We detect 72% of Class 0 objects from this sample
and 31% of Class I objects. No starless cores are detected. We use the flux
densities measured from these data to improve constraints on the correlations
between radio luminosity and bolometric luminosity, infrared luminosity and,
where measured, outflow force. We discuss the differing behaviour of these
objects as a function of protostellar class and investigate the differences in
radio emission as a function of core mass. Two of four possible very low
luminosity objects (VeLLOs) are detected at 1.8 cm.Comment: 18 pages, 9 figures, accepted MNRA
SunyaevâZelâdovich observations with AMI of the hottest galaxy clusters detected in the XMMâNewton Cluster Survey
We have obtained deep SunyaevâZelâdovich (SZ) observations towards 15 of the hottest XMM Cluster Survey (XCS) clusters that can be observed with the Arcminute Microkelvin Imager (AMI). We use a Bayesian analysis to quantify the significance of our SZ detections. We detect the SZ effect at high significance towards three of the clusters and at lower significance for a further two clusters. Towards the remaining 10 clusters, no clear SZ signal was measured. We derive cluster parameters using the XCS mass estimates as a prior in our Bayesian analysis. For all AMI-detected clusters, we calculate large-scale mass and temperature estimates while for all undetected clusters we determine upper limits on these parameters. We find that the large-scale mean temperatures derived from our AMI SZ measurements (and the upper limits from null detections) are substantially lower than the XCS-based core-temperature estimates. For clusters detected in the SZ, the mean temperature is, on average, a factor of 1.4 lower than temperatures from the XCS. Our upper limits on the cluster temperature of undetected systems are lower than the mean XCS derived temperature
AMI Galactic Plane Survey at 16 GHz â I. Observing, mapping and source extraction
The Arcminute Microkelvin Imager (AMI) Galactic Plane Survey is a large-area survey of the outer Galactic plane to provide arcminute resolution images at milli-Jansky sensitivity in the centimetre-wave band. Here we present the ?rst data release of the survey, consisting of 868 deg2 of the Galactic plane, covering the area 76? 170? between latitudes of |b| 5?, at a central frequency of 15.75 GHz (1.9 cm). We describe in detail the drift-scan observations which have been used to construct the maps, including the techniques used for observing, mapping and source extraction, and summarize the properties of the ?nalized data sets. These observations constitute the most sensitive Galactic plane survey of large extent at centimetre-wave frequencies greater than 1.4 GHz
The radio source count at 93.2âGHz from observations of 9C sources using AMI and CARMA
We present results from follow-up observations of a sample of 80 radio sources, originally detected as part of the 15.2-GHz Ninth Cambridge (9C) survey. The observations were carried out, close to simultaneously, at two frequencies: 15.7âGHz, using the Arcminute Microkelvin Imager (AMI) Large Array, and 93.2âGHz, using the Combined Array for Research in Millimeter-wave Astronomy (CARMA).
There is currently little direct information on the 90-GHz-band source count for S âČ 1âJy. However, we have used the measured 15.7-to-93.2-GHz spectral-index distribution and 9C source count to predict the differential source count at 93.2âGHz as 26 ± 4(S/Jy)^(â2.15)âJy^(â1) sr^(â1); our projection is estimated to be most accurate for 10 âČ S âČ 100 mJy.
Our estimated differential count is more than twice the 90-GHz prediction made by Waldram et al.; we believe that this discrepancy is because the measured 43-GHz flux densities used in making their prediction were too low. Similarly, our prediction is significantly higher than that of Sadler et al. at 95âGHz. Since our spectral-index distribution is similar to the 20-to-95-GHz distribution measured by Sadler et al. and used in making their prediction, we believe that the difference is almost entirely attributable to the dissimilarity in the lower frequency counts used in making the estimates